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Smeary bands in gel electrophoresis


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#1 dnasequencer

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Posted 22 December 2014 - 08:41 AM

I have a new sets of primers, so am checking them with conventional PCR before proceeding with my work. When running my PCR products  on 2% agarose gel, I have got smeary bands even for the DNA ladder. What could be the reason for this?

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#2 phage434

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Posted 22 December 2014 - 10:24 AM

Did you accidentally make the gel with water instead of  buffer?



#3 dnasequencer

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Posted 22 December 2014 - 10:33 AM

No, I used 1% TAE buffer to prepare the gel and to run the gel as well

#4 dnasequencer

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Posted Yesterday, 02:16 AM

I figured out my mistake, it was the DNA volume which is too high. I have got sharp bands when decreasing the total volume of DNA added to the wells.






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