I have a new sets of primers, so am checking them with conventional PCR before proceeding with my work. When running my PCR products on 2% agarose gel, I have got smeary bands even for the DNA ladder. What could be the reason for this?
Smeary bands in gel electrophoresis
Posted 22 December 2014 - 10:24 AM
Did you accidentally make the gel with water instead of buffer?
Posted 22 December 2014 - 10:33 AM
Posted Yesterday, 02:16 AM
I figured out my mistake, it was the DNA volume which is too high. I have got sharp bands when decreasing the total volume of DNA added to the wells.