I could really use some help here and would appreciate any direction!
I have been trying for a while to detect BDNF protein (mature form is 14kda as a monomer).
It is present in very low levels in skeletal muscle. Since muscle contains many other larger and more abundant proteins, I would like to fractionate the muscle homogenate to retain proteins below ~50kda. How can I do this?
Is there anything similar to the chemical method of acetonitrile for removing albumin from serum?
Any thoughts on this?
Does anyone know of any other method, chemical or physical? As BDNF is susceptible to degradation, I would prefer to try something that more crudely removes large proteins in less steps (rather than more thoroughly in more steps). Just something to get that smaller protein fraction of muscle homogenate to enhance the proportion of BDNF...
Thank you, thank you for any suggestions!