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miRNA yield from human urine

miRNA urine circulating miRNAs rna quality microRNA

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#1 itsron

itsron

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Posted Yesterday, 08:28 PM

I recently isolated miRNA from human urine using Quigen miRNeasy serum/plasma kit (which works with urine as well) and quantified using NanoDrop. Even from freshly isolated sample, I am getting a horrible 260/280 ratios like 1.5 and 1.6. Concentration is around 8-30 ng/ul

 

My feeling is that the choice of Nanodrop is not right as miRNAs are only present in lower amounts in urine and these levels may not be detected accurately by NanoDrop. I can not imagine another reason for such a low ratio (I am pretty sure I did the experiment right).

 

Has anybody here worked with urinary miRNAs? I would like to know how much is the concentration range you are getting after isolation, and how to you quantify/qualify miRNAs?( I guess it would be Bioanalyzer).

 

Thanks mates!

 


Edited by itsron, Yesterday, 08:30 PM.






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