Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

TE buffer pH adjustment

Molecular biology TE buffer Tris EDTA DNA Extraction DNA isolation

  • Please log in to reply
1 reply to this topic

#1 Md Jibran Alam

Md Jibran Alam


  • Active Members
  • Pip
  • 8 posts

Posted 18 December 2014 - 09:48 AM

I've lowered the pH of TE buffer less than 8.0 by mistake while adding HCL. Then I've added 1N NaOH to raise the pH. Would it make any problem while preserving the DNA?

#2 phage434



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,747 posts

Posted 18 December 2014 - 10:02 AM

Not a problem for the DNA, but you have effectively increased the salt concentration of the TE. This could be an issue for downstream uses (including elution from columns) if there is a LOT of salt.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.