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TE buffer pH adjustment

Molecular biology TE buffer Tris EDTA DNA Extraction DNA isolation

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#1 Md Jibran Alam

Md Jibran Alam


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Posted 18 December 2014 - 09:48 AM

I've lowered the pH of TE buffer less than 8.0 by mistake while adding HCL. Then I've added 1N NaOH to raise the pH. Would it make any problem while preserving the DNA?

#2 phage434



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Posted 18 December 2014 - 10:02 AM

Not a problem for the DNA, but you have effectively increased the salt concentration of the TE. This could be an issue for downstream uses (including elution from columns) if there is a LOT of salt.

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