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band obove the ladder

electrophoresis page

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3 replies to this topic

#1 Perindu Bulan

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Posted 17 December 2014 - 02:19 AM

why the band above the ladder?why the other have no band?

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#2 mdfenko


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Posted 17 December 2014 - 07:24 AM

what did you do?


what are we looking at (don't say "an agarose gel")?

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#3 JDSBlueDevl



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Posted 10 January 2015 - 11:24 AM

It looks like there might be some contamination in the marker.  A band toward the top of the lane like that indicates a large DNA size.  I would run it again with a fresh marker not previously used.




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Posted 15 January 2015 - 08:44 AM

That band is a large DNA. Not a contamination though.


Ladders are usually made by cutting a large DNA with specific restriction enzymes, so if there was some undigested DNA left over (and sometimes it is deliberately partially digested, to get the desired pattern from a tandem repeat large DNA), it shows up as that. 


I have never put an ant in the agarose gel well. I wish at least she had run  dry.png

Edited by CPRES, 15 January 2015 - 09:00 AM.

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