i have tried 3 times to send for sequencing of bacteria gene. this gene i have cloned into T-easy vector. and then i sent it to company for checking the sequence is wrong or correct. but even 3 times , i got wrong all of them. in sequence missed 1 or 2 oligonucleotide at different site in gene.
Does anyone know the reason? i need your help.
i would so much appreciate your comment
Edited by Huongnguyen.miss, 17 December 2014 - 01:55 AM.