I am doing bac to bac transformation of DH10Bac.
Blue white screening was used to select the transformed colony.
White colony was picked and used for colony pcr and re-streak on new selective plate.
The PCR result showed no amplification at all, while the colony re-streak on new selective plate still white.
The transformation protocol, competent cell, and PCR reagent and protocol were same batch with my previous experiment which I manage to amplify my plasmid.
I did tried to purify the plasmid and analyze using gel electrophoresis, and this gave me positive result, however, the extracted plasmid still can't be amplified by PCR reaction.
* The condition of all PCR reagents had been tested using my lab mates samples, and their samples can be amplified (but with faint band).