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Plasmid Methylation


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#1 Raygoza

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Posted 14 December 2014 - 09:13 PM

Hi, does anyone know if there exist a commercial enzyme or protocol to remove methylation of dna extracted from a cell? one method is PCR but it's not very efficient in terms of yield when we are talking of plasmids.


Edited by Raygoza, 14 December 2014 - 09:14 PM.


#2 bob1

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Posted 15 December 2014 - 01:10 AM

The easiest way to do this is to transform them into a methylation deficient strain of E coli, or propagate them in one to start with.



#3 Raygoza

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Posted 15 December 2014 - 05:35 AM

This is what i'm doing now, but i'm trying to see if there are alternative ways.



#4 bob1

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Posted 15 December 2014 - 09:54 PM

I don't recall any DNA demethylases that are available commercially, they certainly won't be cheaper than methylation deficient bugs.






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