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Question about MBP purification


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#1 skepticalguy99

skepticalguy99

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Posted 12 December 2014 - 08:20 AM

Hi,

 

I'm currently working with a MBP-fusion to my protein of interest.  I'm mostly curious about the resin that is utilized for purification.  After expression in E.coli, purification on amylose resin is very common.  However, the resin begins to degrade after a few uses and I'm wondering if anyone knows any more sustainable alternatives?  I've heard about using a His-Tag as the main purification method, but I had also looked at Dextrin Sepharose resins.  The problem is I haven't had any luck with a literature search on Dextrin stability in the presence of the amylases in E.coli extracts.  Any help or suggestions would be greatly appreciated!






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