I only have two possible restriction sites for cloning an insert into my vector. One end is a sticky end (EcoRI) and the other end is a blunt end (PmeI or MssI). I have never done a blunt end ligation. Can I ligate my insert in a single reaction with these two different type of restriction sites? Are the reaction conditions for sticky and blunt ends ligation compatible for single reaction? Has anyone done this before? Or would this combination make my insert incompatible using these two restriction sites?