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Detection of Td-Tomato protein in frozen sections


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#1 Anana

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Posted 09 December 2014 - 05:07 AM

Hello,

We are working with  Wnt1-cre:Rosatomato mice and have trouble detecting red fluorescence in frozen sections. Any explanation, any tips? 

Thanks a lot



#2 bob1

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Posted 09 December 2014 - 09:38 AM

What processes are you doing to the sections before viewing?



#3 Rsm

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Posted 16 December 2014 - 06:40 AM

fixation before freezing kills most, if not all, fluorescence of GFP etc, interfering with the resonance of the beta barrel. I'd recommend antibody staining against tdT, there are some from Clontech for example.


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