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Quantification of IgG

Immunology

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3 replies to this topic

#1 laminemm

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Posted 04 December 2014 - 02:47 AM

Can I Quantify IgG with this labwares:

 

-Multiskan

-Spectrophotometer Jenway 6315

 

And why i can process?

 

THANKS



#2 mdfenko

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Posted 05 December 2014 - 05:27 AM

you should be able to determine protein concentration. if the igg is pure then yes. if it is a mixture then you will not be able to specifically determine igg.


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#3 laminemm

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Posted 07 December 2014 - 11:48 PM

Thank!

 

How can i purify my IgG?

Have you a protocol?

 

Best regards!



#4 mdfenko

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Posted 08 December 2014 - 05:30 AM

here are a couple of ways:

 

immobilized protein a or protein g will reversibly bind igg (which one you need depends on the species in which the antibody is produced)

 

i used a synthetic protein a and g functional mimic, called avid al, which i purchased from the nest group. unfortunately, they don't seem to carry it anymore (the producing company, bioprobes int'l, inc, appears to be defunct).

 

either way, you bind at neutral pH and elute at low pH (the specific protocol should come with the material purchased).


Edited by mdfenko, 08 December 2014 - 05:32 AM.

talent does what it can
genius does what it must
i do what i get paid to do





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