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can RIPA buffer use to prepare nuclear protein?


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#1 nnchu

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Posted 17 July 2004 - 01:05 AM

:rolleyes:
i want nuclear protein and cytoplast protein.

are there any good suggestin?
thank you

#2 nnchu

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Posted 09 August 2004 - 04:16 PM

please help me!
RIPA:
50mM Tris-cl
1%NP40
0.5 % deoxycholic acid
0.1% SDS
1mM EDTA
150mM NaCl

after 19000*g,15min, i get supernatant,is the supernatant include the whole nucleus protein?




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