can anyone help me with the MTT assay
I am using three types of plant extracts from different plants against MCF-7 cell line,the problem is i got cell viability higher with the extracts (extract+ cell) than without extracts ( cell+ media ). The formula that used foe cell viability is ( OD of extract+cell)/ OD of cell+ media) x 100.
is it possible that all three extracts from different plant enhance the cancer cell ?. I have repeated the assay three time and i got same results.
thank you in advance