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qPCR error bars - confused!

qRT-PCR error bars qPCR statistics

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#1 toffee89

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Posted 26 November 2014 - 06:27 AM

Hi,

 

I need help with some qRT-PCR error bar calculations. I have carried out all of my qPCR with my gene of interest and of my housekeeping gene and have calculated my fold-changes for my gene of interest with the delta-delta-Ct calculation. However putting error bars on my graph showing the fold-changes is confusing me. What data should I be using to calculate this? I can't use the normalized Ct values as this is not correct for fold-changes and when I convert my Ct values (for my calibrator and experimental) using the delta-delta-Ct calculation and then get the SE for that my error bars are bigger than my fold-change. Can anybody shed some light on this and where I am going wrong? I can't use the software that comes with the qPCR machine as it is getting confused with how my plates are arranged.  

 

Thank you in advance!  



#2 Trof

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Posted 19 December 2014 - 03:17 PM

Just curious, what software comes with your machine (i.e. what is your machine) that gets confused by plate arrangement?


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