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How to choose cell line

cell line

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#1 ckzimase



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Posted 25 November 2014 - 07:42 PM

I'm just working with cell line,( prostate cancer) for my anticancer project. I just wonder how do peoples choose the right cell line for research? i read from some journals the type of cell that usually been used, but what the characteristics that is important to look at? from my reading, i choose adherent cell line (DU 145, PC-3 and LNCAP). The characteristic that i most like are of course easy to grow, non resistant to apoptosis (LNCAP is resistant) and can work well in my research.

#2 bob1


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Posted 25 November 2014 - 11:57 PM

The most essential thing is that the system you are using is a model for the thing that you are studying. In the case of prostate cancer, you should use a prostate cancer cell line, preferably one as near to "normal" prostate cancer as you can get. However, cell lines are almost never "normal" as the conditions they are cultured under don't really mimic the conditions in the body, so in that case, choose one that fits your model (which ones express cellular markers that you want, or, undergo processes that you want to study?).

#3 animodel123



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Posted 10 February 2015 - 10:49 PM

If you are going to study prostate cancer, the first thing you need to consider about is to choose a suitable transgenic animal model. Transgenic mice provide an ideal animal model system to study the molecular basis of transformation of normal prostatic cells and the factors influencing the progression to metastatic prostate cancer.

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