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polymerase with or without proofreading activity used in study nucleotide polymo

proofreading activity polymorphism

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#1 kimanh

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Posted 24 November 2014 - 08:38 PM

Dear all! 

I am studying of polymorphism in a gene of fungi. I cloned gene by phusion polymerase and sequencing. Result showed that gene contain 2 polymorphism. But F12 sequence lack 1 nucleotide C. I'm not sure ploymerase removed C???? 

Thanks for your help^^

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#2 bob1

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Posted 24 November 2014 - 11:12 PM

There is no way to tell - if you repeat the amplification and sequencing and get the same result then it is very unlikely that it was the result of a polymerase error. Phusion has 3'-5' exonuclease activity so it is pretty good at fixing errors it generates.







Also tagged with one or more of these keywords: proofreading activity, polymorphism

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