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#1 Chomolungma

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Posted 19 November 2014 - 07:06 PM

Hi all, can any one can tell the basis of detection of anti tumour activity of a certain compound/crude extract?



#2 bob1

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Posted 19 November 2014 - 11:25 PM

The simplest is a cell count - proliferation inhibition.



#3 Chomolungma

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Posted 02 December 2014 - 11:28 PM

Thanks. But other than the cell count, are there any specific genes in cancer cells whose expression level can tell whether it's inhibited or not?



#4 bob1

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Posted 03 December 2014 - 12:15 AM

Yes and no - it depends on the cell type and mode of action of the inhibition. If for instance the drug stimulates apoptosis, then you could look for cleaved Parp or caspases, but if the drug causes senescence, then you could look for upregulation of B-galactosidase.






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