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about microrna overexpressing


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#1 adamos1945

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Posted 19 November 2014 - 06:48 PM

hello

i have a question about overexpressing microrna using pcDNA 3.1+. i cloned the pri-microrna into the plasmid and transfected in the cell lines but i didnt have overexpressing compared to the control.

can u please tell me which is the best vector to overexpress microrna?

thanks.



#2 itsron

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Posted 22 December 2014 - 02:46 AM

Late by a month, but may be this and this will be of some help to you.


Edited by itsron, 22 December 2014 - 02:46 AM.


#3 adamos1945

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Posted 06 January 2015 - 03:11 AM

thank you for your help






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