I need to ask something but I apology in advance if this kind of question was asked here before.
I need to make 10 ml of 5 X Loading buffer which required final concentration 250mM Tris HCL. I have 1 M Tis HCL stoke.
Using C 1 V1 = C2 V2 equation . I found out I need to take 2.5 ml of 1 M Tis HCL stoke to have 250 mM final concentration.
I am wondering what I did it right or I can not dilute molarity and I have to make a stock solution with 250 mM.