Commercial elisa kit was used to measure IgA in pig saliva. Now how can I calculate Absorbance LOD and concentration LOD from my results. The calibration curve is 4 parametric. In additon, mean OD at zero calibrator is 0.06 and SD 0.0045. Thank you in advance for your valuable suggestions.
How to calculate LOD of elisa
Posted 24 November 2014 - 08:02 AM
Generally, the LOD OD = (mean OD at zero calibrator) + 3 x SD.
The LOD concentration can be calculated from the 4PL curve by plugging in the LOD OD calculated above.
Posted 25 November 2014 - 12:38 PM
Posted 26 November 2014 - 05:26 AM
It seems your standard curve range is broad, which can result in a high y-intercept. You can either fix bottom asymptote value to the background value or rerun the std curve in a narrower range to get a better fit in the low concentration range.
Posted 26 November 2014 - 10:31 AM
Posted 26 November 2014 - 03:04 PM
I meant you should try running a different std curve with lower concentration values.
As far as changing the bottom asymptote value, it depends what software you are using.