Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

What to do with a sequence from EST.

EST Gene characterization.

  • Please log in to reply
No replies to this topic

#1 tracylauren.smith



  • Members
  • Pip
  • 1 posts

Posted 18 November 2014 - 12:43 PM

Hi there,

I am a grad student working in an area that's new to me. If I had a sequence of about 400 bases from an expressed sequence tag that someone has sent me what are the first steps I should take to characterize that gene. I am trying to wrap my head around what to do first. Can I run all mRNA from my cells of interest in a northern blot then cut out the band to get the mRNA? If I did this what can I use for a probe? Can I use the sequence I do have to make a primer for PCR to make a probe?


Thanks I am obviously very confused,


Also tagged with one or more of these keywords: EST, Gene characterization.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.