I am a grad student working in an area that's new to me. If I had a sequence of about 400 bases from an expressed sequence tag that someone has sent me what are the first steps I should take to characterize that gene. I am trying to wrap my head around what to do first. Can I run all mRNA from my cells of interest in a northern blot then cut out the band to get the mRNA? If I did this what can I use for a probe? Can I use the sequence I do have to make a primer for PCR to make a probe?
Thanks I am obviously very confused,