Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Non-pH-sensitive fluorophore for relative protein quantification

pH fluorophore FITC albumin protein

  • Please log in to reply
1 reply to this topic

#1 edlowry

edlowry

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 18 November 2014 - 09:35 AM

Hey there.
I'm conducting in vitro drug release studies utilizing FITC-tagged proteins to quantify (relative) release from Float-a-Lyzer dialysis tubes into a dialysate of neutral PBS. 
The problem I'm encountering stems from the pH-sensitive nature of FITC; my control and experimental solutions are at a pH range from 4 to 7.5, so my data isnt representative of actual protein release. 
I'm looking for a non pH-sensitive fluorophore to label these proteins (or other model proteins of ~70kDa) to circumvent this misrepresentation.
I'd really appreciate some input from the community.

Thanks,
Ethan L.

Undergraduate Research Assistant
Laboratory for Vaccine and Immunotherapy Delivery
University of Arkansas



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,827 posts
413
Excellent

Posted 18 November 2014 - 12:25 PM

Try looking through the molecular probes handbook (online at  lifetechnologies.com).







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.