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Non-pH-sensitive fluorophore for relative protein quantification

pH fluorophore FITC albumin protein

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#1 edlowry



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Posted 18 November 2014 - 09:35 AM

Hey there.
I'm conducting in vitro drug release studies utilizing FITC-tagged proteins to quantify (relative) release from Float-a-Lyzer dialysis tubes into a dialysate of neutral PBS. 
The problem I'm encountering stems from the pH-sensitive nature of FITC; my control and experimental solutions are at a pH range from 4 to 7.5, so my data isnt representative of actual protein release. 
I'm looking for a non pH-sensitive fluorophore to label these proteins (or other model proteins of ~70kDa) to circumvent this misrepresentation.
I'd really appreciate some input from the community.

Ethan L.

Undergraduate Research Assistant
Laboratory for Vaccine and Immunotherapy Delivery
University of Arkansas

#2 bob1


    Thelymitra pulchella

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Posted 18 November 2014 - 12:25 PM

Try looking through the molecular probes handbook (online at  lifetechnologies.com).

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