I wish to use/create food-grade expression system for lactic acid bacteria for expressing some genes of my interest into them. There are several such expression systems already described in the published papers. Although I have lot of experience in cloning and recombinant expression of genes, probably because of lot of reading I am totally confused, have lost the track and stuck in between. My doubts are as following
1) Where can I get the plasmids from? Can I directly request the authors who have constructed them? If they give it to me and using them if I construct my own plasmids with some really useful genes having some commercial apllication and if I patent such new plasmids/recombinant strains, would there be any copyright/IP- related issues with the authors who shared the original plasmid with me?
2) Would it be a good idea to start de novo: to isolate naturally occuring plasmid in the species/strain that I am interested in and to generated a cloning verctor out of it for my purpose of food-grade expression?
3) If I just want some replication genes from these plasmids, can I simply amplify them from these plasmids so as to not have any copyrigth issue?
4) Is it posssible to contruct a new plasmid vector by amplifing the replication genes and Ori from one vector, selection gene from the other and add MCS by gene synthesis?
(The reason why I want to have my own expression system is (1) only few expression systems avaialble commercially (2) they are costly (3) If I develop a new expression system, that itself would be a good piece of work (4) I can patent such vector and put n-number of genes of my interest into it probably having nnumerous different applications)
Any other related suggestion welcome!!
Edited by rmbio, 18 November 2014 - 02:48 AM.