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Tissue homogenization using Potassium phosphate buffer

Tissue Homogenization Lysis mitochondria Potassium phosphate buf

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#1 MadE for research

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Posted 17 November 2014 - 08:57 PM

Hi Friends,

 

I want to know one thing that, mostly we will be using Potassium phosphate buffer for homogenization to measure enzyme activity in tissue.

My question is whether this homogenization procedure ruptures all the organelles like mitochondria, Golgi etc.

Can we use this protocol to measure activity of mitochondrial enzymes?

 

Thank you.



#2 mdfenko

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Posted 21 November 2014 - 04:42 AM

that depends on the constitution of the lysis buffer and the homogenization conditions (physical as well as chemical).


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