I am doing my masters thesis. My research requires isolation of bacteria from colonic mucosal specimen (from colonoscopy) and collecting mRNA from them. But due to delivery problem, I dont have my mRNA isolation kit and cDNA synthesis kit yet. I have collected the specimens in PBS and now kept in 4'C. I cant preserve the tissues now in ethanol due to the reason that the bacteria with the sample will die. Will the bacteria survive and have a functional mRNA if the tissues stored at -80'C with 80% glycerol?
A crucial part of my study is to collect the mRNA from bacteria without culturing. I also want to collect the DNA from the tissues.