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THP-1 polarization

#THP-1 # ELISA #PMA

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#1 Sabarezkh

Sabarezkh

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Posted 11 November 2014 - 04:17 PM

Hey,
I have started working with THP-1 cells.and have found different protocols regarding the duration of PMA treatment and then polarization to M1 and M2 macrophages. I got weird results with ELISA for TNF-alpha and IL-10. I have tried different concentrations of PMA from 20 nM to 200, and duration of 1 day to 3 days. Some people say that we have to add cytokines while PMA is present and some not. Has anyone a robust protocol?
 
Thanks in advance,
Saba





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