I am currently co-culturing cells on glass cover slips placed in 24-well plates. They were incubated with BrdU 24h before they were fixed in 100% methanol.
After fixation, I was told to let the methanol dry and then place everything in -20C until I was ready to stain (and that's what I did). However, online, I read that the cells must be placed in 4C in PBS so that it doesn't dry.
Could someone explain this discrepancy?