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HELP. Methanol fixation then store in???

methanol fixation

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#1 Jessie Tao

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Posted 06 November 2014 - 07:10 PM

Hi everyone,

 

I am currently co-culturing cells on glass cover slips placed in 24-well plates. They were incubated with BrdU 24h before they were fixed in 100% methanol.

 

After fixation, I was told to let the methanol dry and then place everything in -20C until I was ready to stain (and that's what I did). However, online, I read that the cells must be placed in 4C in PBS so that it doesn't dry.

Could someone explain this discrepancy?

 

Thanks!



#2 bob1

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Posted 06 November 2014 - 08:20 PM

The methanol will dehydrate the cells anyway, they can be then re-hydrated by adding PBS.  You can store them in PBS for a week or so, but not much longer.







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