Hey all, I just have a question about the NEB Tm calculator as I plan on using their Q5 high fidelity polymerase.
Here is the link to the calculator: http://tmcalculator.neb.com/#!/
Where it asks for primer concentration I assume that, with my 10uM/L working primer concentration, the nM value will be 10000nM? Is this correct or have I used the wrong primer concentration?
Any help regarding this would be greatly appreciated.