I'm using BioRad's kaledioscope protein standard, which transferred beautifully. I became aware of this problem after staining for my gene of interest (which did not reveal a band) followed by tubulin staining with a known antibody that works well with westerns (also, no band). So I dried the PVDF and added a few mLs of Invitrogen's SimplyBlue and let sit for 10 minutes, and there are no evident protein bands.
The gel I ran my samples in was a precast 10% bis tris w/ MES buffer. I did not trim any lanes before or after transfer, except for the top of the wells. I did a protein quant via BCA, which gave me ~7ug/uL (turned purple almost instantly). The lysate is not vicous, so I don't think it's clumping up at the top of the well. The obvious next step is to just run the gel and do a coomassie on it, but what could possibly be the issue?