Posted 14 July 2004 - 06:44 AM
Have you got any idea?
Posted 14 July 2004 - 09:10 AM
Posted 14 July 2004 - 10:18 AM
A common problem with insert digested from PCR product is the efficiency of digestion. Sometimes the digestion simply doesn't work even you have enough extra bases outside the restriction site. In this case, first do a TA cloning, then cut the insert from the vector and subclone it.
Edited by labrat, 14 July 2004 - 10:50 AM.