1 reply to this topic

[prishah]

Hi,

 

I am running an experiment which gives thick gel bands on 1%agarose gels with TBE buffer which causes difficulties in determining band size.

 

My samples vary from 100-900ng. Right now, I am using 5ul sample and 15ul Master mix containing digestive enzymes (0.5ul each) , 2 ul suitable NEB buffer and rest water for all the samples.

 

Any suggestion on improving results would be welcome.I need a standazided method which can give me good results.

 

 

Thanks,

Priyanka

Edited by prishah, 27 October 2014 - 08:28 AM.

[GNANA]

You might have to reduce your sample for gel run and use comb with narrow/thinner wells.