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Gel Bands


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#1 prishah

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Posted 27 October 2014 - 08:27 AM

Hi,

 

I am running an experiment which gives thick gel bands on 1%agarose gels with TBE buffer which causes difficulties in determining band size.

 

My samples vary from 100-900ng. Right now, I am using 5ul sample and 15ul Master mix containing digestive enzymes (0.5ul each) , 2 ul suitable NEB buffer and rest water for all the samples.

 

Any suggestion on improving results would be welcome.I need a standazided method which can give me good results.

 

 

Thanks,

Priyanka


Edited by prishah, 27 October 2014 - 08:28 AM.


#2 GNANA

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Posted 27 October 2014 - 10:38 AM

You might have to reduce your sample for gel run and use comb with narrow/thinner wells.


I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....




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