For the double-stranded DNA fragment below, give the sequences you would expect for probes labelled by a) random priming, b ) end-filling of EcoRI sites or c) end-labelling. Circle all nucleotides in the probes that will have incorporated radioactive phosphorus.
5′-GCAGAATTCGATTAGCAATGGACTGGGCCAAATTGCGAATTCCAATA-3′
3′-CGTCTTAAGCTAATCGTTACCTGACCCGGTTTAACGCTTAAGGTTAT-5′
a) Probe made by random priming with the hexameric primers TGGAAT AND GATTAG:
b ) Probe made by end-filling of EcoRI sites:
c) Probe made by end-labelling:
Obvious homework... moved. Bob
Edited by bob1, 24 October 2014 - 01:27 AM.