I am again asking for a word of advice.
I am supposed to be doing fermentations soon. My problem is I never really had much experiences with these.
I need to run an easy fermentation like this.
Yeast DSM 1333
Yeast + Gram positive contaminant (Lactobacillus fermentum)
Yeast + Gram negative contaminant (E.coli)
I decided to use these conditions for 3 fermentations
Broth= Water 1L, yeast extract 10g/L, glucose 20g/L, peptone 20g/L
pH = 6
suddenly my starter culture didnt even grow. Im using aeration now to see how it goes.
My doubt resides here I want to end with contaminants of 1.0x10^5 ufc/ml at least and final yeast of 1.0x10^8ufc/mL. how do I achieve that?
I know I have to use an hemacytometer to determine the amount of yeast initialy but what volume should i pour to start the fermentation?
and same thing for my bacteria... I have OD600=2 for both bacteria. what math do I do to know the volume I need to pour initialy as well and start fermentations?
Lets say I counted my yeast 1.2x10^5 ufc/mL. And if I start fermentation at 10^5 ufc/ml what volume should I pour? Is it by following the formula
initial concentration x volume = final concentration (start fermentation) x volume of fermentation (250mL) would this be right?
but what is the formula to find out this wanted volume for the bacteria in OD=2 (can I use the hemacytometer to count bacteria? isnt that going to be really hard to do?)
Thank you for your time