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normalization of qPCR results


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#1 FCS scholar

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Posted 22 October 2014 - 02:35 AM

Hi all, 

I have used SYBR method to quantify the copy numbers from sediment samples. I extracted DNA from sediments, optimized the PCR reactions and the standard curve was made with a cloned plasmid (uidA gene and 16s rRNA). Now I am looking to normalize my uidA gene copies to the 16s copy numbers.

Is it simply to divide the uidA gene copies/16s copy numbers from the same samples and can I say the uidA gene copies were normalized to the 16s copy numbers ??

for example I have x gene copies of uidA gene/ng and y gene copies of 16s/ng from the same sample, so x/y will be the normalized copy numbers if uidA to the 16s ??

Any suggestions, 

Thanks






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