Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

normalization of qPCR results

  • Please log in to reply
No replies to this topic

#1 FCS scholar

FCS scholar


  • Members
  • Pip
  • 1 posts

Posted 22 October 2014 - 02:35 AM

Hi all, 

I have used SYBR method to quantify the copy numbers from sediment samples. I extracted DNA from sediments, optimized the PCR reactions and the standard curve was made with a cloned plasmid (uidA gene and 16s rRNA). Now I am looking to normalize my uidA gene copies to the 16s copy numbers.

Is it simply to divide the uidA gene copies/16s copy numbers from the same samples and can I say the uidA gene copies were normalized to the 16s copy numbers ??

for example I have x gene copies of uidA gene/ng and y gene copies of 16s/ng from the same sample, so x/y will be the normalized copy numbers if uidA to the 16s ??

Any suggestions, 


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.