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How to retrieve precursor miRNA sequence with extra bases for cloning for olive


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#1 adamos1945

adamos1945

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Posted 21 October 2014 - 11:37 PM

hello i want to clone the precursor sequence of pol-mir-122 of olive flounder, so i want to retrieve the precursor miRNA of the desired microrna i wanted to follow the steps in this topic http://www.protocol-...es-for-cloning/ but couldn't find the full genome of olive flounder.

can please someone help me to retrieve the sequence?

it is a crucial step in my project thanks in advance


Edited by adamos1945, 21 October 2014 - 11:51 PM.





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