Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Selectively arresting a subpopulation of biotinylated yeast cells.

yeast

  • Please log in to reply
4 replies to this topic

#1 yeastresearcher22

yeastresearcher22

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 21 October 2014 - 07:55 AM

Hi everyone,

 

One of the experiments that my lab is working on right now involves liquid cultures of yeast cells, where a subpopulation is biotinylated.  We are looking for a method to target these biotinylated yeast cells, and selectively arrest/kill them, while leaving the rest of the (non-biotinylated) cells alone, and we are a bit stumped.  Has anyone ever attempted anything like this?  Any ideas or suggestions would be very much appreciated.  

 

Thanks,

 

YeastResearcher22



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,407 posts
517
Excellent

Posted 21 October 2014 - 11:40 AM

Could you isolate the biotinylated yeast by passing them through a streptaviding column of some sort?



#3 yeastresearcher22

yeastresearcher22

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 22 October 2014 - 07:30 AM

Unfortunately not - it all has to occur in the suspended culture.  

 

I was thinking I could perhaps use a streptavidin-conjugated small fungicidal peptide, or something like that?



#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,407 posts
517
Excellent

Posted 22 October 2014 - 12:01 PM

Hmmm, that might work if you can prevent the other cell from taking it up. You could do this by masking the antibiotic somehow - perhaps something that would only be internalized in close proximity to the surface of the cell, or only has a lethal dose above a certain dose which could only be achieved by the biotinylated yeast?.



#5 yeastresearcher22

yeastresearcher22

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 22 October 2014 - 02:12 PM

Yes, something like that was what I was thinking.  Thank you.  

 

If you or anyone else happen to have any other ideas, please let me know! :)







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.