Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cell viability with PI staining

PI

  • Please log in to reply
2 replies to this topic

#1 cell 293

cell 293

    member

  • Active Members
  • Pip
  • 15 posts
1
Neutral

Posted 20 October 2014 - 06:55 PM

Hi, 

 

I want to test the cell viability by PI.

 

But i have only found the protocol of cell cycle.

 

In the protocol, it have to add 70% EtOH to fix cell and add RNase A with PI staining solution.

 

Should i do the steps if i just want to test viability?

 

Should i do a trypam blue stain and visually counting to compare it?

 

Thanks a lot.



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,722 posts
399
Excellent

Posted Yesterday, 12:22 AM

PI is not transported across viable cell membranes, so it will only penetrate dead/non-viable cells. Fixing your cells will kill them and, seeing as you are using ethanol, will probably permeabilize the membranes such that PI will penetrate. As a viability stain, you wouldn't fix your cells, but I'm not sure exactly the process.



#3 Tabaluga

Tabaluga

    Making glass out of shards

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 392 posts
49
Excellent

Posted Yesterday, 03:03 AM

To check viability, just add PI to your sample immediately before measuring. Dead cells will take it up immediately so you can then discriminate live and dead populations on FACS.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.