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cell viability with PI staining


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#1 cell 293

cell 293


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Posted 20 October 2014 - 06:55 PM



I want to test the cell viability by PI.


But i have only found the protocol of cell cycle.


In the protocol, it have to add 70% EtOH to fix cell and add RNase A with PI staining solution.


Should i do the steps if i just want to test viability?


Should i do a trypam blue stain and visually counting to compare it?


Thanks a lot.

#2 bob1


    Thelymitra pulchella

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Posted 21 October 2014 - 12:22 AM

PI is not transported across viable cell membranes, so it will only penetrate dead/non-viable cells. Fixing your cells will kill them and, seeing as you are using ethanol, will probably permeabilize the membranes such that PI will penetrate. As a viability stain, you wouldn't fix your cells, but I'm not sure exactly the process.

#3 Tabaluga


    Making glass out of shards

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Posted 21 October 2014 - 03:03 AM

To check viability, just add PI to your sample immediately before measuring. Dead cells will take it up immediately so you can then discriminate live and dead populations on FACS.

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Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.


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