I'm using a SIINFEKL and Trp2 tetramers from a relatively large company; however, every time I do a double staining with CD8 my CD8+ Tetramer -ve population shifts into the alternate channel. This is not an issue with compensation, as I am using PE and APC, which shouldn't overlap. What alternatives are there to the tetramer?
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Having issues with tetramers, what are alternatives
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