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Isolation of whole cell membrane including proteins, lipids etc.


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#1 arshimalikk

arshimalikk

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Posted 16 October 2014 - 09:42 AM

Hi,
I wish to isolate total outer membrane (including proteins, lipids etc.) from mouse cells and later extract any ncRNA attached to the membrane, if any, for RNA-Seq. Can any one suggest me any protocol to isolate whole cell membrane in such a way that if any ncRNA attached to it is not degraded and could later be extracted through simple RNA extraction methods. Thanks





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