Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Can anyone recommend some immunoprecipitation kits?

Protein antibody immunoprecipitation

  • Please log in to reply
1 reply to this topic

#1 ionchannelbk

ionchannelbk

    member

  • Active Members
  • Pip
  • 10 posts
1
Neutral

Posted 16 October 2014 - 09:42 AM

Hello,

 

I will try to immunoprecipitate (IP) some small peptide from the insect blood (haemolymph). I have no IP experience. Can anyone recommend some immunoprecipitation kits to me?

 

Thanks!



#2 miST32

miST32

    member

  • Active Members
  • Pip
  • 28 posts
0
Neutral

Posted 12 November 2014 - 04:58 PM

I find Invitrogen/Life's protein A and protein G Dynabeads to be pretty reliable and easy to use.  Not exactly a kit, but the buffers you find in most kits are very simple to prepare (and much cheaper).

Usually all you need for a straightforward IP is:
1)  A good solid phase (I recommend Dynabeads and a magnet over agarose/sepharose slurry with centrifugation)
2) A simple IP buffer (NaCl between 100-300mM, 50mM Tris, and 0.1-1% Triton or NP40, pH 7.4 is a good start - adjust for your system).
3) A clean and verified antibody

With those it's just a matter of optimizing your antibody amount and IP method.

All that said, I had some decent luck with the Pierce magnetic direct IP/CoIP kit.  Overall though, I don't think it performs any better than a home-made IP system.

Good luck!


Edited by miST32, 12 November 2014 - 04:59 PM.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.