My question is trivial but I am carrying out primers reconstitution and just want to be sure to avoid mistakes.
Exemple for one primer:
according to my primer synthesis repport, I sould add 129µl of water to obtain a 100µM solution (stock tube).
Then I should prepare 100 µl of a 1:10 solution =10µM=10picomol/µl....right?
I have to take 10 µl of my stock solution and add 90ml to obtain a 10µM solution (second tube)
To perform my PCR, I need my primer at 6µM, then I did calculation as in first time
I need 60µl from the second tube and add 40µl of water to obtain my working tube at 6µM and then ready to go
Is it right or there is any mistake there?
thank's in advance