Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

MAPK/MEK Inhibitor stored incorrectly! Is it still ok?

MAPK/MAK enzyme

  • Please log in to reply
2 replies to this topic

#1 matthew.summers

matthew.summers

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 13 October 2014 - 07:18 PM

Hi All,

 

My lab recently purchased 100mg (as a powder) of the MAPK/MEK inhibitor PD0325901. It is recommended to store at -20 for up to 3 months. 

The problem is I only just realised for the last 4 weeks it has been incorrectly stored at 4*C in our normal lab fridge. 

 

Does anybody have an idea how stable this inhibitor is, and if this period at 4*C would have reduced its function?

 

Thanks for any tips!

Matt. 



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,831 posts
415
Excellent

Posted 14 October 2014 - 03:44 PM

The usual advice in these sorts of situations would be to throw it out, but you could test it and see.

 

The throwing out is because, if you doubt something now, how will it affect your results later? Would you trust any result you get using a reagent that you have doubts about?



#3 matthew.summers

matthew.summers

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 19 October 2014 - 03:17 PM

Thanks for the reply.

 

In case others are interested, In the end I contacted the manufacturer and they assured me that the compound is stable at 4*C for at least a month, and that for storage any longer, -20 /-80 is recommended. 






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.