Hi I obtained Antibody purification kit(protein G) and developed my lysed serum (mouse serum is red due to have hemoglobin ) but my cartridge do not passed my sample and we washed and eluted our sample in upper of cartridge. of course, we used only 100µl of resin . we think the hemoglobin is problem.
1-plz help me about treatment way of serum method ?
2-could we reuse this cartridge or If not , may i used another cartridge ,whatever.