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protein G

mouse serum lysed serum protein G chromatography IgG purification

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#1 soltan

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Posted 13 October 2014 - 08:56 AM

Hi I obtained Antibody purification kit(protein G) and developed my lysed serum (mouse serum is red due to have hemoglobin ) but my cartridge do not passed my sample and we washed and eluted our sample in upper of cartridge. of course, we used only 100µl of resin . we think the hemoglobin is problem.

1-plz help me about treatment way of serum method ? unsure.png 

2-could we reuse this cartridge or If not , may i used another cartridge ,whatever. wacko.png 

yours Faithfullyrolleyes.gif 



#2 mdfenko

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Posted 14 October 2014 - 04:08 AM

the hemoglobin should not affect binding of the antibody to protein g, you may have to wash more to remove all of it before you elute the antibody.

 

protein g should be reusable as long as you don't use overly harsh conditions to elute the bound antibody.

 

are you sure you need protein g and not protein a to bind your antibody?

 

have you determined that the antibody bound to the protein g and didn't just elute in the wash?


talent does what it can
genius does what it must
i do what i get paid to do





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