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transfection efficiency


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#1 aditya

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Posted 12 July 2004 - 10:33 PM

i want to calculate transfection efficiency.

the expt is: i plated 10(6) cells, used calcium phosphate method, transfected GFP plasmid. after 7 days on G418 selection, how do i calculate the efficiency of transfection based on amount of DNA used and the cell number plated ?
plz sueegst me any book or weblink which will give me a n answer.

#2 weimin42

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Posted 13 July 2004 - 01:41 AM

Hi ,aditya


I have abtained one student sheet for calculating tansfection efficiency and hope it help you.Would you like gave me your E-mail address?

weimin42




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