Hi. My Product size is 405bp. I have isolated RNA from mouse epididymis and i converted RNA to cDNA by using reverse transcriptese (Applied Biosystems). from the first time, i got amplification of my gene (405bp). After that i could n't found reproducibility. I dont know the exact problem. Then i had changed two more set primer from different company. Till now i couldn't solve my problem. Please suggest me to troubleshoot go get amplification. here i attached my gel picture.
Edited by rubhadevi, 11 October 2014 - 01:05 AM.