I am trying to figure out what is going on with my human adipose-derived stem cell culture purchased from Lonza. One issue is what seems to be a fungal contamination. I have attached two images of the cell culture under the microscope and of the flask (image 1 and 2). If I seem to have a fungal contamination, do I need to go through everything are sterilize it? What do you all consider to be the best method to clear this issue up?
Additionally, I seem to have an odd cell shape to some of the adipose-derived stem cells in some of the cell cultures (image 3). It seems to be overtaking a large number of the cells. I don't believe there's any possible way it could be contamination from another cell-line because I never change media or passage cells in conjunction with other cell types. Is this typical behavior for adipose-derived stem cells?
Thank you for your help!