Good morning forum scientists,
I am working with a species of Gram-negative bacteria which has both an alpha and beta hemolytic phenotype (complete clearing of blood below and around colonies.)
I would very much like to isolate and identify which protein/s are responsible for the beta-hemolytic phenotype and to characterize the system involved in its production/processing.
I am wondering if anyone has experience in Beta-hemolysins from bacteria?
I am considering native-page and zymograms with blood/erytrocytes (if even possible?), mass spec on isolated proteins from the clear agar zones themselves? But perhaps this is the wrong way to go about this. In liquid culture less beta-hemolysin is produced.
If someone had protocols for characterisation etc of these B-H's that would be tremendously helpful.
A genomic approach has identified some homologs to hemolysins and I am in the process of cloning for expression. However, approaching this at the protein level is also advised.
Thanks in advance
Edited by Chris22, 08 October 2014 - 07:22 AM.