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How to treat HEK293 with S9 fraction?

HEK293

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#1 cell 293

cell 293

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Posted 07 October 2014 - 06:59 PM

Hi, everyone

 

In my experiment, i need to treat HEK293 cells with drug including S9 fraction.

 

In the protocol of S9. The medium including S9 have to remove and wash with PBS after treating in 4 hours.

 

But the adhesion ability of HEK293 cells are very week.

 

The cells will be lost if i wash them.

 

What can i do to improve it?

 

Thanks a lot.







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